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Published Reports & Past Projects

Listed here are published reports and presentations resulting from our various projects and research over the years. Also included are project descriptions.


Pioneered the development of undersea habitats, equipment, and techniques to live and work on the ocean floor.

Living and Working In The Sea,

Miller and Koblick. Five Corners Publication, 2nd edition, 1995.

"Effect on vital capacity of saturation in normoxic hyperbaric environment." Peterson R.E., Hamilton R.W., Olstad C.S., Undersea Biomedical Research, 1993.

"Spurious increase in vital capacity by lung 'packing'." Hamilton R.W., Olstad C.S., Peterson R.E., Undersea Biomedical Research, 1993.

"Ethanol and venous bubbles after decompression in humans." Eckenoff R.G., Olstad C. S., Undersea Biomedical Research, 1991.

"Time for saturation in humans as defined by venous gas bubbles." Eckenoff R.G., Olstad C.S., Undersea Biomedical Research, 1991.

"Effect on vital capacity of saturation in a noxmoric hyperbaric environment." Peterson R.E., Hamilton R.W., Olstad C.S., Feingold J.A., Report under NOAA Contract NA88AA-D-UR063, May 1991.

"Gender effect on venous bubble formation after decompression from prolonged 16 fswg exposures." Eckenoff R.G., Olstad C.S., Undersea Biomedical Research, 1990.

"Human dose-response relationship foe decompression and endogenous bubble formation." Eckenoff, R.G., Olstad C.S., Carrod G.E., Journal of Applied Physiology, 1990.

"Venous gas emboli in humans after prolonged exposure to 1.48 ATA (16 fswg) air." Eckenoff T.G., Olstad C.S., Carrod G.E., Undersea Biomedical Research, 1989.

"An Analysis of Saturation Diving by Scientists." Olsed, D.A. Marine Technology Society Journal, Vol 11, No. 5, June 1976.

"Down Deeper on Air." Koblick, IG, Sea Technology Magazine, Vol 16, No. 7, July 1975.

"A Study of Puerto Rico Coral Reef Systems." Olsen, D.A> and Sheen, M.O. Hydro-Lab Journal, Vol. 3, No 1, 1975.

"Cost-effectiveness of Saturation Diving as a Scientific Data Acquisition Tool." Olsen, D.A. PRINUL Special Report No. 5, 1973.


MRDF's work in fisheries research and aquaculture provided valuable data for ongoing projects.

NASA CELSS OCEAN Project (hydroponics). An operational Controlled Ecologicial Life Support System (CELSS) protoype hydrophonis unit planned for lunar, Mars or space station missions field tested in the MarineLab Undersea Laboratory. Chamberland D., John F. Kennedy Space Center Office of Biological Research and Life Support report, May 1994.

"Population Dynamics, Ecology and Behavior of Spiny Lobsters of St. John, U.S. Virgin Islands." Olsen D.A., Herrnkind W.A., and Cooper R.A., Bulletin of Southern California Academic Sciences, 1972.

"Growth and Mortality of Spiny Lobsters of St. John, U.S. Virgin Islands," Olsen D.A., Herrnkind W.A. and Koblick I. G., PRINUL Special Report, 1972.


Developed the technique for controlled extraction of calcium carbonate from seawater for "marine accretion projects."

Chris Scala, artist. Accretion of several scultpures in the MRDF using this technique. These sculptures included a statue of a woman, "Thanks," subsequently used in a fountain (1989); a sculpture called "Exploding Sphere" which was displayed at the 1994 World Cup; and a statue named "Ascending Swimmer," a larger-than-life female figure (1996.)

Michele Oka Doner, artist. Accretion of two obelisks which were then placed in a park in Santa Monica, CA. (1992)

"The Venice Accretion Project." Michele Oka Doner; A Kress Foundation Publication, 1991.


Helped plan and implement management and conservation of marine resources. 

"Results of a Workshop on Coral Reef Research and Management in the Florida Keys: A Blueprint for Action." Miller, J., Editor, National Undersea Research Program Research Report 88-5, 1988.

"Undersea Labs for Marine Resources Inventory." Koblick I. G., Biaggi, J.V., Olsen, D.A. and Geiger, E.M., Marine Technology Society Journal, Vol. 8, No. 1, 1974.

"Siltation Experiments in Corals, in Situ." Kolehmainen, S., Science, 1974

"Oxygen Balance of Tropical Benthic Communities." Wells, J.M. and Olsen D.A., Proc., Xth. Mtg. Associated Island Marine Biology Lab of the Caribbean, 1973

"Benthic Algal Ecology in a Deep Reef and Sand Habitat Off Puerto Rico." Dahl, A.L., Journal of Marine Botany, 1973.

"Cleaning Symbiosis in Puerto Rico with Comparison to the Tropical Pacific." Losey, G., Copiea, 1973.


Remote Operation of ROV's over the Internet for Education and Research, Chris Olstad (MRDF) and Tom Glebas (VideoRay); published in Proceedings, Underwater Intervention 2008.

CISTERN ARCHEOLOGICAL MAPPING EXPEDITION TO MALTA, USING MICRO-ROV’S (Project Update) Investigators: Christopher Clark, Computer Science Dept, Cal. Polytech State U. San Luis Obispo; Christopher Olstad, Marine Resources Development Foundation, Key Largo, FL.; Keith Buhagiar, Dept. of Classics & Archeology, University of Malta; Timmy Gambin, AURORA Special Purpose Trust, Malta. 2008.

EVALUATION OF NEURAL NETWORKS FOR DATA CLASSIFICATION, RECOGNITION, AND NAVIGATION IN THE MARINE ENVIRONMENT. (Doctoral Dissertation) Author: Brian P. Howell. Department of Marine and Environmental Systems, Florida Institute of Technology, May, 2004.

EXTRACTION EFFICIENCY OF AN ARTIFICIAL GILL Investigators: Bruce Zenner, Bob Cashon, Dan Rittschof, Taylor (Tap) Pryor
A collaborative investigation between Duke University and Aquanautics Corporation to observe carrier gas oxygen extraction efficiency thru a semipermeable membrane, and the filtration fouling aspects of this seawater system, during deployment from a manned undersea station. August 1984.


Scott Carpenter Space Analog Station. The Analog station was constructed at Kennedy Space Center and placed in the MRDF lagoon as an analog for space exploration, providing K-12 students with a better understanding of life support systems and crew functions in space. 1998.


"Diving in a Drop of Water." Egner, Sarah. Alert Diver Magazine, Q1 Winter 2015.

"Mangroves and Seagrass." Egner, Sarah. Alert Diver Magazine, Spring 2014.


Facilitated research and helped establish medical expertise for Man in the Sea programs.

THE EFFECTS OF HYPERBARIC ENVIRONMENT-INDUCED pH CHANGES ON THE GENERATION TIMES OF PSEUDOMONASAERUGINOSA (Abstract) Authors: Contessa E. Creager, Sarah 0.Hammar, Mitchell W. Jacobs, John Wayne Kalda, Spring J. Mayer, Brandon J. McCall, Jenny M. Volkert, William J. Soeffing. 2002 Proceedings of the South Dakota Academy of Science, Vol. 81, p. 267.

OBSERVATIONS ON THE COLONIAL MORPHOLOGIES, CELL-CELL ADHESION CHARACTERISITCS AND ANTIBIOTIC SENSITIVITY OF PSEUDOMONAS AERUGINOSA IN A HYPERBARIC ENVIRONMENT (Abstract). Authors: Brandon J. McCall, Sarah O. Hammar, Nicolas L. Strasser, William J. Soeffing. 2002 Proceedings of the South Dakota Academy of Science, Vol. 81, p 269.

(Abstract / Presentation) Authors: Sarah O. Hammar, Brandon J. McCall, Jenny M. Volkert, Contessa E. Creager, Spring Mayer, Nicholas L. Strasser, Julie M. Altmann, Julie A. Groen, Casey J. Schlenker, William J. Soeffing. 2001 Proceedings of the South Dakota Academy of Science, Vol. 80, p.435

THE EFFECTS OF HYPERBARIC AIR ON THE POPULATION GROWTH RATES OF THE COMMON ETIOLOGICAL AGENTS OF OTITIS AND THE DEVELOPMENT OF A BUFFERED GENERAL GROWTH MEDIUM FOR USE IN COMPRESSED AIR ENVIRONMENTS (Abstract / Presentation) Authors: Bradley L. Dubbels, Jena D. French, Jennifer D. Tranas, Richard T. Dickey, David J. Dingsor, William J. Soeffing. 1996 Proceedings of the South Dakota Academy of Science, Vol. 75, p.149.

"Antibiotic sensitivity testing at hyperbaric conditions (1.62 ATA).' Bacon J.J., Iverson D.A., Wortham G.M., Sioux Falls University. Presented at 1993 National Academy of Sciences Meeting.

"Seawater exchange systems to provide life support in manned undersea habitats." Olstad C.S., MRDF Internal Report, September 1992.

"Susceptibility of divers in open water to motion sickness." Norfleet W.T., Peterson R.E., Hamilton R.W., Olstad C.S., Undersea Biomedical Research, 1992.

"The changing population distribution of coliform, psuedomenal and staphloccal species inhabiting the external auditory canal, as they respond to hyperbaric incubation periods between frequent marine diving exposures." Tieszen K.K., Sioux Falls University, Hyperbaric Studies Sect- Marine Microbiology report, May 1991.

"The efficacies of common antibiotics administered at 1.62 ATA to two escherichia coli-variants." Kuske S.G., Sioux Falls University, Hyperbaric Studies Sect-Marine Microbiology report, May 1991.